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antibodies against collagen 4  (SouthernBiotech)


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    Structured Review

    SouthernBiotech antibodies against collagen 4
    Antibodies Against Collagen 4, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibodies+against+collagen+4/Goat+Anti-Type+IV+Collagen-Alexa+Fluor+488/us12440479-289-6-10
    Average 93 stars, based on 11 article reviews
    antibodies against collagen 4 - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Staining:

    Article Title: Prevention or treatment of chronic organ injury
    Article Snippet: For immunofluorescent staining, primary antibodies against ARNT (5537S, Cell Signaling, Danvers, USA), Hif1α (H6535, Sigma, St. Louis, USA), Hif2α (ab20654, Abcam Biochemicals, Cambridge, UK), phosphorylated Smad1/5/8 (pSmad1/5/8, sc-12353, Santa Cruz Biotechnology, Dallas, USA), CD45 (550539, BD Biosciences, Franklin Lakes, USA), Collagen-1 (ab34710, Abcam Biochemicals, Cambridge, UK), α-smooth muscle actin (aSMA, A5228, Sigma, St. Louis, USA), FKBP12 (ab2918, Abcam Biochemicals, Cambridge, UK) and YY1 (ab12132, Abcam Biochemicals, Cambridge, UK) were used, secondary antibodies were labeled with Alexa Fluor 488 or 568 (Life Technologies, Carlsbad, USA). .. Renal basement membranes were stained with antibodies against Collagen-4 (1340-30, SouthernBiotech, Birmingham, USA), cardiac cell membranes with antibodies against WGA (W11261, Life Technologies, Carlsbad, USA). .. Nuclear staining was performed using 4′,6-diamidino-2-phenylindole (DAPI, Vector Laboratories, Burlingame, USA).

    Article Title: Treatment of aberrant fibroblast proliferation
    Article Snippet: For morphometric analysis of interstitial fibrosis in mice and human biopsies, we assessed the fibrotic area using cellSens (Olympus, Tokyo, Japan) software. .. Renal basement membranes were stained with antibodies against Collagen-4 (1340-30, SouthernBiotech, Birmingham, USA). .. Nuclear staining was performed using 4′,6-diamidino-2-phenylindole (DAPI, Vector Laboratories, Burlingame, USA).

    Whole Genome Amplification:

    Article Title: Prevention or treatment of chronic organ injury
    Article Snippet: For immunofluorescent staining, primary antibodies against ARNT (5537S, Cell Signaling, Danvers, USA), Hif1α (H6535, Sigma, St. Louis, USA), Hif2α (ab20654, Abcam Biochemicals, Cambridge, UK), phosphorylated Smad1/5/8 (pSmad1/5/8, sc-12353, Santa Cruz Biotechnology, Dallas, USA), CD45 (550539, BD Biosciences, Franklin Lakes, USA), Collagen-1 (ab34710, Abcam Biochemicals, Cambridge, UK), α-smooth muscle actin (aSMA, A5228, Sigma, St. Louis, USA), FKBP12 (ab2918, Abcam Biochemicals, Cambridge, UK) and YY1 (ab12132, Abcam Biochemicals, Cambridge, UK) were used, secondary antibodies were labeled with Alexa Fluor 488 or 568 (Life Technologies, Carlsbad, USA). .. Renal basement membranes were stained with antibodies against Collagen-4 (1340-30, SouthernBiotech, Birmingham, USA), cardiac cell membranes with antibodies against WGA (W11261, Life Technologies, Carlsbad, USA). .. Nuclear staining was performed using 4′,6-diamidino-2-phenylindole (DAPI, Vector Laboratories, Burlingame, USA).



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    A. Representative images of MDA-MB-231 cells expressing FUCCI cell cycle reporters and F-actin marker mCerulean3-Lifeact7 in G1 (left, invadopodia marked by yellow arrowhead), early S (middle) and S/G2/M (right) phases. Scale bar 10 µm. B. ECM degradation (degraded spot marked by yellow arrowhead) by invadopodia of the cell in A (left). C. Representative traces of red- and green- fluorescence of one MDA-MB-231-FUCCI cell over 3600 minutes. Brackets indicate how the cell cycle phases are classified based on the fluorescence signal: early S phase corresponds to the period where red fluorescence has a negative slope. The ratio of mean to maximum gray values is presented. D. Percentage of cells in each phase of the cell cycle in the Migration state (M, left bar) and the Invadopodia state (I, right bar) in an asynchronous population. Cells in the Migration state were defined by cell translocation and absence of invadopodia. Cells in the Invadopodia state were identified by the presence of actin puncta and degradation spots in the underlying gelatin layer. See Video S1, S2. E. Representative image of a 3D spheroid at 0h and 24h after embedding in high-density <t>collagen</t> <t>I</t> (5 mg/ml). Leading cells of the invasive strand are marked by white arrowheads. Scale bar 100 µm. See Video S3. F. Representative image of an invasive strand in a 3D spheroid at 72h after embedding in high-density collagen (5 mg/ml) merged (left) and individual channels showing FUCCI and degraded collagen antibody staining <t>(Col1-¾C,</t> right panel). Scale bar 50 µm.
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    Image Search Results


    A. Representative images of MDA-MB-231 cells expressing FUCCI cell cycle reporters and F-actin marker mCerulean3-Lifeact7 in G1 (left, invadopodia marked by yellow arrowhead), early S (middle) and S/G2/M (right) phases. Scale bar 10 µm. B. ECM degradation (degraded spot marked by yellow arrowhead) by invadopodia of the cell in A (left). C. Representative traces of red- and green- fluorescence of one MDA-MB-231-FUCCI cell over 3600 minutes. Brackets indicate how the cell cycle phases are classified based on the fluorescence signal: early S phase corresponds to the period where red fluorescence has a negative slope. The ratio of mean to maximum gray values is presented. D. Percentage of cells in each phase of the cell cycle in the Migration state (M, left bar) and the Invadopodia state (I, right bar) in an asynchronous population. Cells in the Migration state were defined by cell translocation and absence of invadopodia. Cells in the Invadopodia state were identified by the presence of actin puncta and degradation spots in the underlying gelatin layer. See Video S1, S2. E. Representative image of a 3D spheroid at 0h and 24h after embedding in high-density collagen I (5 mg/ml). Leading cells of the invasive strand are marked by white arrowheads. Scale bar 100 µm. See Video S3. F. Representative image of an invasive strand in a 3D spheroid at 72h after embedding in high-density collagen (5 mg/ml) merged (left) and individual channels showing FUCCI and degraded collagen antibody staining (Col1-¾C, right panel). Scale bar 50 µm.

    Journal: bioRxiv

    Article Title: Invadopodia degrade ECM in the G1 phase of the cell cycle

    doi: 10.1101/412916

    Figure Lengend Snippet: A. Representative images of MDA-MB-231 cells expressing FUCCI cell cycle reporters and F-actin marker mCerulean3-Lifeact7 in G1 (left, invadopodia marked by yellow arrowhead), early S (middle) and S/G2/M (right) phases. Scale bar 10 µm. B. ECM degradation (degraded spot marked by yellow arrowhead) by invadopodia of the cell in A (left). C. Representative traces of red- and green- fluorescence of one MDA-MB-231-FUCCI cell over 3600 minutes. Brackets indicate how the cell cycle phases are classified based on the fluorescence signal: early S phase corresponds to the period where red fluorescence has a negative slope. The ratio of mean to maximum gray values is presented. D. Percentage of cells in each phase of the cell cycle in the Migration state (M, left bar) and the Invadopodia state (I, right bar) in an asynchronous population. Cells in the Migration state were defined by cell translocation and absence of invadopodia. Cells in the Invadopodia state were identified by the presence of actin puncta and degradation spots in the underlying gelatin layer. See Video S1, S2. E. Representative image of a 3D spheroid at 0h and 24h after embedding in high-density collagen I (5 mg/ml). Leading cells of the invasive strand are marked by white arrowheads. Scale bar 100 µm. See Video S3. F. Representative image of an invasive strand in a 3D spheroid at 72h after embedding in high-density collagen (5 mg/ml) merged (left) and individual channels showing FUCCI and degraded collagen antibody staining (Col1-¾C, right panel). Scale bar 50 µm.

    Article Snippet: For immunofluorescence, the spheroids were fixed in 4% paraformaldehyde for 20 min, permeabilized in 0.1% TritonX-100 for 20 min, blocked in 1% FBS/1% BSA in PBS overnight at 4°C, incubated with primary antibody against degraded collagen I (Col1-C 3 / 4 , ImmunoGlobe, 1:100) overnight at 4°C and with secondary antibody for 3-4 hours at room temperature.

    Techniques: Expressing, Marker, Fluorescence, Migration, Translocation Assay, Staining